The Lid/KDM5 histone demethylase complex activates a critical effector of the oocyte-to-zygote transition

English version

Autoři: Daniela Torres-Campana ^aff001; Shuhei Kimura ^aff002; Guillermo A. Orsi ^aff001; Béatrice Horard ^aff001; Gérard Benoit ^aff001; Benjamin Loppin ^aff001
Působiště autorů: Laboratoire de Biologie et de Modélisation de la Cellule, CNRS UMR5239, Ecole Normale Supérieure de Lyon, University of Lyon, France ^aff001; Laboratoire de Biométrie et Biologie Evolutive, Université Lyon 1, CNRS, UMR 5558, Villeurbanne F-69622, France ^aff002
Vyšlo v časopise: The Lid/KDM5 histone demethylase complex activates a critical effector of the oocyte-to-zygote transition. PLoS Genet 16(3): e32767. doi:10.1371/journal.pgen.1008543
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pgen.1008543

Souhrn

Following fertilization of a mature oocyte, the formation of a diploid zygote involves a series of coordinated cellular events that ends with the first embryonic mitosis. In animals, this complex developmental transition is almost entirely controlled by maternal gene products. How such a crucial transcriptional program is established during oogenesis remains poorly understood. Here, we have performed an shRNA-based genetic screen in Drosophila to identify genes required to form a diploid zygote. We found that the Lid/KDM5 histone demethylase and its partner, the Sin3A-HDAC1 deacetylase complex, are necessary for sperm nuclear decompaction and karyogamy. Surprisingly, transcriptomic analyses revealed that these histone modifiers are required for the massive transcriptional activation of deadhead (dhd), which encodes a maternal thioredoxin involved in sperm chromatin remodeling. Unexpectedly, while lid knock-down tends to slightly favor the accumulation of its target, H3K4me3, on the genome, this mark was lost at the dhd locus. We propose that Lid/KDM5 and Sin3A cooperate to establish a local chromatin environment facilitating the unusually high expression of dhd, a key effector of the oocyte-to-zygote transition.

Klíčová slova:

Drosophila melanogaster – Embryos – Fertilization – Gene expression – Histones – Chromatin – Ovaries – Sperm

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