Effect of CdCl2 and CuSO4 on the Production of Flavonoids by the Culture of Ononis arvensis L. in vitro
Vliv CdCl2 a CuSO4 na produkci flavonoidů kulturou Ononis arvensis L. in vitro
Byl sledován vliv abiotických elicitorů – CuSO4 a CdCl2 na produkci flavonoidů v kalusové kultuře Ononis arvensis po 24; 48 a 168 hodinové aplikaci. Testované elicitory ovlivňovaly výrazně produkci flavonoidů v této kultuře. Statisticky významný nárůst produkce flavonoidů byl zaznamenán při použití roztoku CdCl2 v koncentraci 0,5 mg.l -1 a koncentraci 0,05 mg.l -1 po 48 hodinách kultivace, u koncentrace 0,005 mg.l -1 po 24 i 48 hodinách kultivace. U roztoku CuSO4 v koncentraci 0,5 mg.l -1po 48 i 168 hodinách. Nejvyšší nárůst hladiny flavonoidů byl zjištěn po elicitaci CdCl2 v koncentraci 0,005 mg.l -1 , kde se produkce zvýšila o 67 % oproti kontrole po 48 hodinách kultivace.
Klíčová slova:
Ononis arvensis – flavonoidy – elicitace – těžké kovy
Authors:
L. Tůmová; R. Rusková
Authors‘ workplace:
Katedra farmakognozie Farmaceutické fakulty Univerzity Karlovy, Hradec Králové
Published in:
Čes. slov. Farm., 1998; , 261-263
Category:
Overview
The effect of the abiotic elicitors CuSO4 and CdCl2 on the production of flavonoids in the callus culture of Ononis arvensis after 24; 48 and 168-hour administration was examined. The tested elicitors markedly influenced the production of flavonoids in this culture. A statistically significant increase in the production of flavonoids was recorded with the use of a CdCl2 solution in a concen- tration of 0.5 mg.l -1 and a concentration of 0.05 mg.l -1 after 48 hours of cultivation, in a concentration of 0.005 mg.l -1 after 24 and 48 hours of cultivation. In a CuSO4 solution in a concentration of 0.5 mg.l -1 it was after both 48 and 168 hours. The highest increase in the level of flavonoids was found after elicitation of CdCl2 in a concentration of 0.005 mg–l -1
Key words:
Ononis arvensis – flavonoids – elicitation – heavy metals
Labels
Pharmacy Clinical pharmacologyArticle was published in
Czech and Slovak Pharmacy
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