Cardiac Snail family of transcription factors directs systemic lipid metabolism in Drosophila


Autoři: Ying Liu aff001;  Hong Bao aff001;  Weidong Wang aff002;  Hui-Ying Lim aff001
Působiště autorů: Department of Physiology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, United States of America aff001;  Department of Medicine, Section of Endocrinology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, United States of America aff002
Vyšlo v časopise: Cardiac Snail family of transcription factors directs systemic lipid metabolism in Drosophila. PLoS Genet 15(11): e32767. doi:10.1371/journal.pgen.1008487
Kategorie: Research Article
doi: 10.1371/journal.pgen.1008487

Souhrn

Maintenance of normal lipid homeostasis is crucial to heart function. On the other hand, the heart is now recognized to serve an important role in regulating systemic lipid metabolism; however, the molecular basis remains unclear. In this study, we identify the Drosophila Snail family of transcription factors (herein termed Sna TFs) as new mediators of the heart control of systemic lipid metabolism. Overexpression of Sna TF genes specifically in the heart promotes whole-body leanness whereas their knockdown in the heart promotes obesity. In addition, flies that are heterozygous for a snail deficiency chromosome also exhibit systemic obesity, and that cardiac-specific overexpression of Sna substantially reverses systemic obesity in these flies. We further show that genetically manipulating Sna TF levels in the fat body and intestine do not affect systemic lipid levels. Mechanistically, we find that flies bearing the overexpression or inhibition of Sna TFs in the postnatal heart only exhibit systemic lipid metabolic defects but not heart abnormalities. Cardiac-specific alterations of Sna TF levels also do not perturb cardiac morphology, viability, lipid metabolism or fly food intake. On the other hand, cardiac-specific manipulations of Sna TF levels alter lipogenesis and lipolysis gene expression, mitochondrial biogenesis and respiration, and lipid storage droplet 1 and 2 (Lsd-1 and Lsd-2) levels in the fat body. Together, our results reveal a novel and specific role of Sna TFs in the heart on systemic lipid homeostasis maintenance that is independent of cardiac development and function and involves the governance of triglyceride synthesis and breakdown, energy utilization, and lipid droplet dynamics in the fat body.

Klíčová slova:

Fats – Gastrointestinal tract – Heart – Hyperexpression techniques – Lipid analysis – Lipid metabolism – Lipids – Mitochondria


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Genetika Reprodukční medicína

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PLOS Genetics


2019 Číslo 11

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