Photodamage repair pathways contribute to the accurate maintenance of the DNA methylome landscape upon UV exposure


Autoři: Stéfanie Graindorge aff001;  Valérie Cognat aff001;  Philippe Johann to Berens aff001;  Jérôme Mutterer aff001;  Jean Molinier aff001
Působiště autorů: Institut de biologie moléculaire des plantes, UPR2357-CNRS, Strasbourg, France aff001
Vyšlo v časopise: Photodamage repair pathways contribute to the accurate maintenance of the DNA methylome landscape upon UV exposure. PLoS Genet 15(11): e32767. doi:10.1371/journal.pgen.1008476
Kategorie: Research Article
doi: 10.1371/journal.pgen.1008476

Souhrn

Plants are exposed to the damaging effect of sunlight that induces DNA photolesions. In order to maintain genome integrity, specific DNA repair pathways are mobilized. Upon removal of UV-induced DNA lesions, the accurate re-establishment of epigenome landscape is expected to be a prominent step of these DNA repair pathways. However, it remains poorly documented whether DNA methylation is accurately maintained at photodamaged sites and how photodamage repair pathways contribute to the maintenance of genome/methylome integrities.

Using genome wide approaches, we report that UV-C irradiation leads to CHH DNA methylation changes. We identified that the specific DNA repair pathways involved in the repair of UV-induced DNA lesions, Direct Repair (DR), Global Genome Repair (GGR) and small RNA-mediated GGR prevent the excessive alterations of DNA methylation landscape. Moreover, we identified that UV-C irradiation induced chromocenter reorganization and that photodamage repair factors control this dynamics. The methylome changes rely on misregulation of maintenance, de novo and active DNA demethylation pathways highlighting that molecular processes related to genome and methylome integrities are closely interconnected. Importantly, we identified that photolesions are sources of DNA methylation changes in repressive chromatin. This study unveils that DNA repair factors, together with small RNA, act to accurately maintain both genome and methylome integrities at photodamaged silent genomic regions, strengthening the idea that plants have evolved sophisticated interplays between DNA methylation dynamics and DNA repair.

Klíčová slova:

Arabidopsis thaliana – DNA damage – DNA methylation – DNA repair – Methylation – Plant genomics – Small interfering RNAs – Ultraviolet C


Zdroje

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Genetika Reprodukční medicína

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PLOS Genetics


2019 Číslo 11

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