An EHBP-1-SID-3-DYN-1 axis promotes membranous tubule fission during endocytic recycling


Autoři: Jinghu Gao aff001;  Linyue Zhao aff001;  Qian Luo aff001;  Shuyao Liu aff001;  Ziyang Lin aff001;  Peixiang Wang aff001;  Xin Fu aff001;  Juan Chen aff001;  Hongjie Zhang aff002;  Long Lin aff001;  Anbing Shi aff001
Působiště autorů: Department of Biochemistry and Molecular Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China aff001;  Faculty of Health Sciences, University of Macau, Avenida da Universidade, Taipa, Macau SAR, China aff002;  Institute for Brain Research, Huazhong University of Science and Technology, Wuhan, Hubei, China aff003;  Key Laboratory of Neurological Disease of National Education Ministry, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China aff004
Vyšlo v časopise: An EHBP-1-SID-3-DYN-1 axis promotes membranous tubule fission during endocytic recycling. PLoS Genet 16(5): e32767. doi:10.1371/journal.pgen.1008763
Kategorie: Research Article
doi: 10.1371/journal.pgen.1008763

Souhrn

The ACK family tyrosine kinase SID-3 is involved in the endocytic uptake of double-stranded RNA. Here we identified SID-3 as a previously unappreciated recycling regulator in the Caenorhabditis elegans intestine. The RAB-10 effector EHBP-1 is required for the endosomal localization of SID-3. Accordingly, animals with loss of SID-3 phenocopied the recycling defects observed in ehbp-1 and rab-10 single mutants. Moreover, we detected sequential protein interactions between EHBP-1, SID-3, NCK-1, and DYN-1. In the absence of SID-3, DYN-1 failed to localize at tubular recycling endosomes, and membrane tubules breaking away from endosomes were mostly absent, suggesting that SID-3 acts synergistically with the downstream DYN-1 to promote endosomal tubule fission. In agreement with these observations, overexpression of DYN-1 significantly increased recycling transport in SID-3-deficient cells. Finally, we noticed that loss of RAB-10 or EHBP-1 compromised feeding RNAi efficiency in multiple tissues, implicating basolateral recycling in the transport of RNA silencing signals. Taken together, our study demonstrated that in C. elegans intestinal epithelia, SID-3 acts downstream of EHBP-1 to direct fission of recycling endosomal tubules in concert with NCK-1 and DYN-1.

Klíčová slova:

Caenorhabditis elegans – Cell membranes – Endosomes – Fluorescence imaging – Gastrointestinal tract – RNA interference – Tyrosine kinases – Vacuoles


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