Trichinella spiralis-induced mastocytosis and erythropoiesis are simultaneously supported by a bipotent mast cell/erythrocyte precursor cell


Autoři: Juan M. Inclan-Rico aff001;  Christina M. Hernandez aff001;  Everett K. Henry aff001;  Hannah G. Federman aff001;  Chandler B. Sy aff001;  John J. Ponessa aff001;  Alexander D. Lemenze aff003;  Nathanael Joseph aff004;  Patricia Soteropoulos aff004;  Aimee M. Beaulieu aff001;  George S. Yap aff001;  Mark C. Siracusa aff001
Působiště autorů: Center for Immunity and Inflammation, New Jersey Medical School, Rutgers-The State University of New Jersey, Newark, New Jersey, United States of America aff001;  Department of Medicine, New Jersey Medical School, Rutgers-The State University of New Jersey, Newark, New Jersey, United States of America aff002;  The Department of Pathology, Immunology and Laboratory Medicine, New Jersey Medical School, Rutgers-The State University of New Jersey, Newark, New Jersey, United States of America aff003;  The Genomics Center, New Jersey Medical School, Rutgers-The State University of New Jersey, Newark, New Jersey, United States of America aff004;  Department of Microbiology, Biochemistry and Molecular Genetics, New Jersey Medical School, Rutgers-The State University of New Jersey, Newark, New Jersey, United States of America aff005
Vyšlo v časopise: Trichinella spiralis-induced mastocytosis and erythropoiesis are simultaneously supported by a bipotent mast cell/erythrocyte precursor cell. PLoS Pathog 16(5): e32767. doi:10.1371/journal.ppat.1008579
Kategorie: Research Article
doi: 10.1371/journal.ppat.1008579

Souhrn

Anti-helminth responses require robust type 2 cytokine production that simultaneously promotes worm expulsion and initiates the resolution of helminth-induced wounds and hemorrhaging. However, how infection-induced changes in hematopoiesis contribute to these seemingly distinct processes remains unknown. Recent studies have suggested the existence of a hematopoietic progenitor with dual mast cell-erythrocyte potential. Nonetheless, whether and how these progenitors contribute to host protection during an active infection remains to be defined. Here, we employed single cell RNA-sequencing and identified that the metabolic enzyme, carbonic anhydrase (Car) 1 marks a predefined bone marrow-resident hematopoietic progenitor cell (HPC) population. Next, we generated a Car1-reporter mouse model and found that Car1-GFP positive progenitors represent bipotent mast cell/erythrocyte precursors. Finally, we show that Car1-expressing HPCs simultaneously support mast cell and erythrocyte responses during Trichinella spiralis infection. Collectively, these data suggest that mast cell/erythrocyte precursors are mobilized to promote type 2 cytokine responses and alleviate helminth-induced blood loss, developmentally linking these processes. Collectively, these studies reveal unappreciated hematopoietic events initiated by the host to combat helminth parasites and provide insight into the evolutionary pressure that may have shaped the developmental relationship between mast cells and erythrocytes.

Klíčová slova:

Bone marrow cells – Flow cytometry – Helminth infections – Helminths – Mast cells – Parasitic diseases – Red blood cells – Stem cells


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