A divergent CheW confers plasticity to nucleoid-associated chemosensory arrays

Autoři: Annick Guiseppi aff001;  Juan Jesus Vicente aff002;  Julien Herrou aff001;  Deborah Byrne aff003;  Aurelie Barneoud aff001;  Audrey Moine aff001;  Leon Espinosa aff001;  Marie-Jeanne Basse aff004;  Virginie Molle aff005;  Tâm Mignot aff001;  Philippe Roche aff004;  Emilia M. F. Mauriello aff001
Působiště autorů: Laboratoire de Chimie Bactérienne, Aix Marseille Univ, CNRS, Marseille, France aff001;  Physiology & Biophysics, University of Washington, Seattle, WA, United States of America aff002;  Protein Purification Platform, Institut de Microbiologie de la Méditerranée, CNRS, Marseille, France aff003;  CRCM, Institute Paoli-Calmettes, CNRS, INSERM, Aix Marseille Univ, Marseille, France aff004;  Laboratoire de Dynamique des Interactions Membranaires Normales et Pathologique, Montpellier II et I University, CNRS, France aff005
Vyšlo v časopise: A divergent CheW confers plasticity to nucleoid-associated chemosensory arrays. PLoS Genet 15(12): e32767. doi:10.1371/journal.pgen.1008533
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pgen.1008533


Chemosensory systems are highly organized signaling pathways that allow bacteria to adapt to environmental changes. The Frz chemosensory system from M. xanthus possesses two CheW-like proteins, FrzA (the core CheW) and FrzB. We found that FrzB does not interact with FrzE (the cognate CheA) as it lacks the amino acid region responsible for this interaction. FrzB, instead, acts upstream of FrzCD in the regulation of M. xanthus chemotaxis behaviors and activates the Frz pathway by allowing the formation and distribution of multiple chemosensory clusters on the nucleoid. These results, together, show that the lack of the CheA-interacting region in FrzB confers new functions to this small protein.

Klíčová slova:

Cell fusion – Cell motility – Crystal structure – Fluorescence microscopy – Histidine – Phosphorylation – Protein structure – Protein structure comparison


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