Modulators of hormonal response regulate temporal fate specification in the Drosophila brain

Autoři: Giovanni Marchetti aff001;  Gaia Tavosanis aff001
Působiště autorů: Dynamics of neuronal circuits, German Center for Neurodegenerative Diseases (DZNE), Germany aff001;  LIMES-Institute, University of Bonn, Germany aff002
Vyšlo v časopise: Modulators of hormonal response regulate temporal fate specification in the Drosophila brain. PLoS Genet 15(12): e32767. doi:10.1371/journal.pgen.1008491
Kategorie: Research Article
doi: 10.1371/journal.pgen.1008491


Neuronal diversity is at the core of the complex processing operated by the nervous system supporting fundamental functions such as sensory perception, motor control or memory formation. A small number of progenitors guarantee the production of this neuronal diversity, with each progenitor giving origin to different neuronal types over time. How a progenitor sequentially produces neurons of different fates and the impact of extrinsic signals conveying information about developmental progress or environmental conditions, on this process represent a key, but elusive questions. Each of the four progenitors of the Drosophila mushroom body (MB) sequentially gives rise to the MB neuron subtypes. The temporal fate determination pattern of MB neurons can be influenced by extrinsic cues, conveyed by the steroid hormone ecdysone. Here, we show that the activation of Transforming Growth Factor-β (TGF-β) signalling via glial-derived Myoglianin regulates the fate transition between the early-born α’β’ and the pioneer αβ MB neurons by promoting the expression of the ecdysone receptor B1 isoform (EcR-B1). While TGF-β signalling is required in MB neuronal progenitors to promote the expression of EcR-B1, ecdysone signalling acts postmitotically to consolidate theα’β’ MB fate. Indeed, we propose that if these signalling cascades are impaired α’β’ neurons lose their fate and convert to pioneer αβ. Conversely, an intrinsic signal conducted by the zinc finger transcription factor Krüppel-homolog 1 (Kr-h1) antagonises TGF-β signalling and acts as negative regulator of the response mediated by ecdysone in promoting α’β’ MB neuron fate consolidation. Taken together, the consolidation of α’β’ MB neuron fate requires the response of progenitors to local signalling to enable postmitotic neurons to sense a systemic signal.

Klíčová slova:

Cell staining – Metamorphosis – Neurons – RNA interference – Vision – Neuroblasts – Hormone receptor signaling


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Genetika Reprodukční medicína

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PLOS Genetics

2019 Číslo 12

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