Kinetochore-associated Stu2 promotes chromosome biorientation in vivo

Autoři: Matthew P. Miller aff001;  Rena K. Evans aff001;  Alex Zelter aff002;  Elisabeth A. Geyer aff003;  Michael J. MacCoss aff004;  Luke M. Rice aff003;  Trisha N. Davis aff002;  Charles L. Asbury aff005;  Sue Biggins aff001
Působiště autorů: Howard Hughes Medical Institute, Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America aff001;  Department of Biochemistry, University of Washington, Seattle, Washington 98195, United States of America aff002;  Departments of Biophysics and Biochemistry, UT Southwestern Medical Center, Dallas, Texas, United States of America aff003;  Department of Genome Sciences, University of Washington, Seattle, Washington, United States of America aff004;  Department of Physiology & Biophysics, University of Washington, Seattle, Washington, United States of America aff005
Vyšlo v časopise: Kinetochore-associated Stu2 promotes chromosome biorientation in vivo. PLoS Genet 15(10): e32767. doi:10.1371/journal.pgen.1008423
Kategorie: Research Article
doi: 10.1371/journal.pgen.1008423


Accurate segregation of chromosomes to daughter cells is a critical aspect of cell division. It requires the kinetochores on duplicated chromosomes to biorient, attaching to microtubules from opposite poles of the cell. Bioriented attachments come under tension, while incorrect attachments lack tension and must be released to allow proper attachments to form. A well-studied error correction pathway is mediated by the Aurora B kinase, which destabilizes low tension-bearing attachments. We recently discovered that in vitro, kinetochores display an additional intrinsic tension-sensing pathway that utilizes Stu2. The contribution of kinetochore-associated Stu2 to error correction in cells, however, was unknown. Here, we identify a Stu2 mutant that abolishes its kinetochore function and show that it causes biorientation defects in vivo. We also show that this Stu2-mediated pathway functions together with the Aurora B-mediated pathway. Altogether, our work indicates that cells employ multiple pathways to ensure biorientation and the accuracy of chromosome segregation.

Klíčová slova:

Auxins – Cell cycle and cell division – Immunoprecipitation – Metaphase – Microtubules – Protein domains – Saccharomyces cerevisiae – Aurora


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