Macrophages fine tune satellite cell fate in dystrophic skeletal muscle of mdx mice

Autoři: Luca Madaro aff001;  Alessio Torcinaro aff002;  Marco De Bardi aff001;  Federica F. Contino aff002;  Mattia Pelizzola aff004;  Giuseppe R. Diaferia aff005;  Giulia Imeneo aff002;  Marina Bouchè aff006;  Pier Lorenzo Puri aff007;  Francesca De Santa aff002
Působiště autorů: IRCCS Fondazione Santa Lucia (FSL), Rome, Italy aff001;  Institute of Biochemistry and Cell Biology (IBBC), National Research Council (CNR), Rome, Italy aff002;  Department of Biology and Biotechnology “Charles Darwin”, Sapienza University of Rome, Rome, Italy aff003;  Center for Genomic Science of IIT@SEMM, Fondazione Istituto Italiano di Tecnologia (IIT), Milan, Italy aff004;  IRCCS European Institute of Oncology (IEO), Milan, Italy aff005;  DAHFMO-Unit of Histology and Medical Embryology, Sapienza University of Rome, Rome, Italy aff006;  Development, Aging and Regeneration Program, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, California, United States of America aff007
Vyšlo v časopise: Macrophages fine tune satellite cell fate in dystrophic skeletal muscle of mdx mice. PLoS Genet 15(10): e32767. doi:10.1371/journal.pgen.1008408
Kategorie: Research Article
doi: 10.1371/journal.pgen.1008408


Satellite cells (SCs) are muscle stem cells that remain quiescent during homeostasis and are activated in response to acute muscle damage or in chronic degenerative conditions such as Duchenne Muscular Dystrophy. The activity of SCs is supported by specialized cells which either reside in the muscle or are recruited in regenerating skeletal muscles, such as for instance macrophages (MΦs). By using a dystrophic mouse model of transient MΦ depletion, we describe a shift in identity of muscle stem cells dependent on the crosstalk between MΦs and SCs. Indeed MΦ depletion determines adipogenic conversion of SCs and exhaustion of the SC pool leading to an exacerbated dystrophic phenotype. The reported data could also provide new insights into therapeutic approaches targeting inflammation in dystrophic muscles.

Klíčová slova:

Cell differentiation – Cell staining – DAPI staining – Mouse models – Muscle differentiation – Nuclear staining – Skeletal muscles – Muscle regeneration


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