Selection and hybridization shaped the rapid spread of African honey bee ancestry in the Americas

Autoři: Erin Calfee aff001;  Marcelo Nicolás Agra aff002;  María Alejandra Palacio aff002;  Santiago R. Ramírez aff001;  Graham Coop aff001;  Marcelo Nicolás Agra aff003;  María Alejandra Palacio aff003
Působiště autorů: Department of Evolution and Ecology and Center for Population Biology, University of California, Davis aff001;  Center for Population Biology, University of California, Davis, California, United States of America aff001;  Instituto Nacional de Tecnología Agropecuaria (INTA), Balcarce, Argentina aff002;  Department of Evolution and Ecology, University of California, Davis, California, United States of America aff002;  Instituto Nacional de Tecnología Agropecuaria (INTA), Balcarce, Argentina aff003;  Facultad de Ciencias Agrarias, Universidad de Mar del Plata, Balcarce, Argentina aff004
Vyšlo v časopise: Selection and hybridization shaped the rapid spread of African honey bee ancestry in the Americas. PLoS Genet 16(10): e32767. doi:10.1371/journal.pgen.1009038
Kategorie: Research Article
doi: 10.1371/journal.pgen.1009038


Recent biological invasions offer ‘natural’ laboratories to understand the genetics and ecology of adaptation, hybridization, and range limits. One of the most impressive and well-documented biological invasions of the 20th century began in 1957 when Apis mellifera scutellata honey bees swarmed out of managed experimental colonies in Brazil. This newly-imported subspecies, native to southern and eastern Africa, both hybridized with and out-competed previously-introduced European honey bee subspecies. Populations of scutellata-European hybrid honey bees rapidly expanded and spread across much of the Americas in less than 50 years. We use broad geographic sampling and whole genome sequencing of over 300 bees to map the distribution of scutellata ancestry where the northern and southern invasions have presently stalled, forming replicated hybrid zones with European bee populations in California and Argentina. California is much farther from Brazil, yet these hybrid zones occur at very similar latitudes, consistent with the invasion having reached a climate barrier. At these range limits, we observe genome-wide clines for scutellata ancestry, and parallel clines for wing length that span hundreds of kilometers, supporting a smooth transition from climates favoring scutellata-European hybrid bees to climates where they cannot survive winter. We find no large effect loci maintaining exceptionally steep ancestry transitions. Instead, we find most individual loci have concordant ancestry clines across South America, with a build-up of somewhat steeper clines in regions of the genome with low recombination rates, consistent with many loci of small effect contributing to climate-associated fitness trade-offs. Additionally, we find no substantial reductions in genetic diversity associated with rapid expansions nor complete dropout of scutellata ancestry at any individual loci on either continent, which suggests that the competitive fitness advantage of scutellata ancestry at lower latitudes has a polygenic basis and that scutellata-European hybrid bees maintained large population sizes during their invasion. To test for parallel selection across continents, we develop a null model that accounts for drift in ancestry frequencies during the rapid expansion. We identify several peaks within a larger genomic region where selection has pushed scutellata ancestry to high frequency hundreds of kilometers past the present cline centers in both North and South America and that may underlie high-fitness traits driving the invasion.

Klíčová slova:

Bees – Europe – Genetic loci – Honey bees – Invertebrate genomics – Latitude – Single nucleotide polymorphisms – South America


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