The Arabidopsis PHD-finger protein EDM2 has multiple roles in balancing NLR immune receptor gene expression


Autoři: Yan Lai aff001;  Xueqing Maggie Lu aff003;  Josquin Daron aff004;  Songqin Pan aff001;  Jianqiang Wang aff001;  Wei Wang aff005;  Tokuji Tsuchiya aff006;  Eric Holub aff007;  John M. McDowell aff005;  R. Keith Slotkin aff004;  Karine G. Le Roch aff003;  Thomas Eulgem aff001
Působiště autorů: Center for Plant Cell Biology, Institute of Integrative Genome Biology, Department of Botany and Plan Sciences, University of California at Riverside, Riverside, CA, United States of America aff001;  College of Life Sciences, Fujian Agricultural and Forestry University, Fuzhou, Fujian, China aff002;  Center for Infectious Disease and Vector Research, Institute of Integrative Genome Biology, Department of Molecular, Cell and Systems Biology, University of California at Riverside, Riverside, CA, United States of America aff003;  Department of Molecular Genetics, The Ohio State University, Columbus, Ohio, United States of America aff004;  Department of Plant Pathology, Physiology, and Weed Science, Virginia Tech, Blacksburg, VA, United States of America aff005;  College of Bioresource Sciences, Nihon University, Kanagawa, Japan aff006;  School of Life Sciences, University of Warwick, Wellesbourne campus, United Kingdom aff007;  Donald Danforth Plant Science Center, St. Louis, Missouri, United States of America aff008;  Division of Biological Sciences, University of Missouri, Columbia, Missouri, United States of America aff009
Vyšlo v časopise: The Arabidopsis PHD-finger protein EDM2 has multiple roles in balancing NLR immune receptor gene expression. PLoS Genet 16(9): e32767. doi:10.1371/journal.pgen.1008993
Kategorie: Research Article
doi: 10.1371/journal.pgen.1008993

Souhrn

Plant NLR-type receptors serve as sensitive triggers of host immunity. Their expression has to be well-balanced, due to their interference with various cellular processes and dose-dependency of their defense-inducing activity. A genetic “arms race” with fast-evolving pathogenic microbes requires plants to constantly innovate their NLR repertoires. We previously showed that insertion of the COPIA-R7 retrotransposon into RPP7 co-opted the epigenetic transposon silencing signal H3K9me2 to a new function promoting expression of this Arabidopsis thaliana NLR gene. Recruitment of the histone binding protein EDM2 to COPIA-R7-associated H3K9me2 is required for optimal expression of RPP7. By profiling of genome-wide effects of EDM2, we now uncovered additional examples illustrating effects of transposons on NLR gene expression, strongly suggesting that these mobile elements can play critical roles in the rapid evolution of plant NLR genes by providing the “raw material” for gene expression mechanisms. We further found EDM2 to have a global role in NLR expression control. Besides serving as a positive regulator of RPP7 and a small number of other NLR genes, EDM2 acts as a suppressor of a multitude of additional NLR genes. We speculate that the dual functionality of EDM2 in NLR expression control arose from the need to compensate for fitness penalties caused by high expression of some NLR genes by suppression of others. Moreover, we are providing new insights into functional relationships of EDM2 with its interaction partner, the RNA binding protein EDM3/AIPP1, and its target gene IBM1, encoding an H3K9-demethylase.

Klíčová slova:

Arabidopsis thaliana – Gene expression – Gene regulation – Genetic loci – Genomics – Plant genomics – Polyadenylation – Retrotransposons


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