Trichoderma reesei XYR1 activates cellulase gene expression via interaction with the Mediator subunit TrGAL11 to recruit RNA polymerase II


Autoři: Fanglin Zheng aff001;  Yanli Cao aff001;  Renfei Yang aff001;  Lei Wang aff001;  Xinxing Lv aff001;  Weixin Zhang aff001;  Xiangfeng Meng aff001;  Weifeng Liu aff001
Působiště autorů: State Key Laboratory of Microbial Technology, Microbial Technology Institute, Shandong University, Qingdao, Shandong, People’s Republic of China aff001
Vyšlo v časopise: Trichoderma reesei XYR1 activates cellulase gene expression via interaction with the Mediator subunit TrGAL11 to recruit RNA polymerase II. PLoS Genet 16(9): e1008979. doi:10.1371/journal.pgen.1008979
Kategorie: Research Article
doi: 10.1371/journal.pgen.1008979

Souhrn

The ascomycete Trichoderma reesei is a highly prolific cellulase producer. While XYR1 (Xylanase regulator 1) has been firmly established to be the master activator of cellulase gene expression in T. reesei, its precise transcriptional activation mechanism remains poorly understood. In the present study, TrGAL11, a component of the Mediator tail module, was identified as a putative interacting partner of XYR1. Deletion of Trgal11 markedly impaired the induced expression of most (hemi)cellulase genes, but not that of the major β-glucosidase encoding genes. This differential involvement of TrGAL11 in the full induction of cellulase genes was reflected by the RNA polymerase II (Pol II) recruitment on their core promoters, indicating that TrGAL11 was required for the efficient transcriptional initiation of the majority of cellulase genes. In addition, we found that TrGAL11 recruitment to cellulase gene promoters largely occurred in an XYR1-dependent manner. Although xyr1 expression was significantly tuned down without TrGAL11, the binding of XYR1 to cellulase gene promoters did not entail TrGAL11. These results indicate that TrGAL11 represents a direct in vivo target of XYR1 and may play a critical role in contributing to Mediator and the following RNA Pol II recruitment to ensure the induced cellulase gene expression.

Klíčová slova:

Cellulases – DNA transcription – Gene expression – Gene regulation – Mycelium – Saccharomyces cerevisiae – Transcriptional control – Yeast


Zdroje

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