Behavior of dicentric chromosomes in budding yeast


Autoři: Diana Cook aff001;  Sarah Long aff001;  John Stanton aff001;  Patrick Cusick aff001;  Colleen Lawrimore aff001;  Elaine Yeh aff001;  Sarah Grant aff001;  Kerry Bloom aff001
Působiště autorů: Department of Biology, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America aff001
Vyšlo v časopise: Behavior of dicentric chromosomes in budding yeast. PLoS Genet 17(3): e1009442. doi:10.1371/journal.pgen.1009442
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pgen.1009442

Souhrn

DNA double-strand breaks arise in vivo when a dicentric chromosome (two centromeres on one chromosome) goes through mitosis with the two centromeres attached to opposite spindle pole bodies. Repair of the DSBs generates phenotypic diversity due to the range of monocentric derivative chromosomes that arise. To explore whether DSBs may be differentially repaired as a function of their spatial position in the chromosome, we have examined the structure of monocentric derivative chromosomes from cells containing a suite of dicentric chromosomes in which the distance between the two centromeres ranges from 6.5 kb to 57.7 kb. Two major classes of repair products, homology-based (homologous recombination (HR) and single-strand annealing (SSA)) and end-joining (non-homologous (NHEJ) and micro-homology mediated (MMEJ)) were identified. The distribution of repair products varies as a function of distance between the two centromeres. Genetic dependencies on double strand break repair (Rad52), DNA ligase (Lif1), and S phase checkpoint (Mrc1) are indicative of distinct repair pathway choices for DNA breaks in the pericentromeric chromatin versus the arms.

Klíčová slova:

Centromeres – DNA repair – DNA replication – Genomics – Homologous recombination – Nucleolus – Polymerase chain reaction – Dicentric chromosomes


Zdroje

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