Correction: Genome-Wide Reprogramming of Transcript Architecture by Temperature Specifies the Developmental States of the Human Pathogen Histoplasma
Authors:
Sarah A. Gilmore; Mark Voorhies; Dana Gebhart; Anita Sil
Published in the journal:
Correction: Genome-Wide Reprogramming of Transcript Architecture by Temperature Specifies the Developmental States of the Human Pathogen Histoplasma. PLoS Genet 17(4): e1009509. doi:10.1371/journal.pgen.1009509
Category:
Correction
doi:
https://doi.org/10.1371/journal.pgen.1009509
The strain G184AR is incorrectly reported as G186AR throughout the paper. The authors have provided corrected versions of Table 1, Figs 1 and 2, and Supporting Information files S1, S3–S6, S9, and S10 Figs that use the correct strain name G184AR.
(A) Histoplasma hyphae and conidia (infectious state) respond to temperature to differentiate into budding yeast cells (parasitic state) inside of a mammalian host. (B) Phylogeny of Histoplasma illustrating the major lineages of this species, which are geographically and genetically distinct. This neighbor-joining tree was adapted from Katsuga et al. [7] and highlights Histoplasma strains selected for comparative transcriptomics (G217B, G184AR, H88, H143). H88 is highlighted in red as the Hc var. duboisii strain selected for transcriptome analysis. (C) Confocal DIC microscopy showing representative images of yeast (37°C) and hyphal (RT) cell morphologies for the 4 Hc strains used for comparative transcriptomics analyses. Scale bar, 20 μm.
(A) Schematic of transcriptome reconstruction method. (B–C) Boxplots of the length of (B) leader regions that were defined as the distance from the 5’ transcript end to the CDS start codon or (C) 3’ UTR regions that were defined as the distance from the CDS stop codon to the 3’ transcript end were plotted for all assembled and predicted transcripts with CDS regions > 0. Boxes, interquartile range (IQR). Whiskers, 1.5*IQR. For ease of viewing, outliers are not displayed. *, indicates that the predicted transcript leader or 3’ UTR length distributions is not visible on this graph. The predicted transcript leader lengths range from 0–1652 bp and the 3’ UTR lengths range from 0–3112 bp in the 4 Hc strains.
Supporting information
S1 Fig [red]
assembled transcripts are longer in length than the predicted transcript models.
S3 Fig [eps]
Novel transcripts exhibit a bias towards small CDS regions.
S4 Fig [red]
Novel transcripts are expressed at low levels.
S5 Fig [a]
exhibits evolutionarily conserved yeast and hyphal transcript expression patterns.
S6 Fig [eps]
Yeast cells express many transcripts encoding small, putative secreted proteins.
S9 Fig [a]
A subset of transcripts exhibit differential sizes of transcript leader regions in yeast and hyphae.
S10 Fig [a]
Transcript leader regions are more commonly extended in yeast cells.
Zdroje
1. Gilmore SA, Voorhies M, Gebhart D, Sil A (2015) Genome-Wide Reprogramming of Transcript Architecture by Temperature Specifies the Developmental States of the Human Pathogen Histoplasma. PLoS Genet 11(7): e1005395. doi: 10.1371/journal.pgen.1005395 26177267
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