Astrovirus replication in human intestinal enteroids reveals multi-cellular tropism and an intricate host innate immune landscape


Autoři: Abimbola O. Kolawole aff001;  Carmen Mirabelli aff001;  David R. Hill aff002;  Sophia A. Svoboda aff001;  Andrew B. Janowski aff003;  Karla D. Passalacqua aff001;  Benancio N. Rodriguez aff001;  Michael K. Dame aff002;  Pamela Freiden aff004;  Ryan P. Berger aff001;  Diem-lan Vu aff005;  Myra Hosmillo aff006;  Mary X. D. O’Riordan aff001;  Stacey Schultz-Cherry aff004;  Susana Guix aff005;  Jason R. Spence aff002;  David Wang aff009;  Christiane E. Wobus aff001
Působiště autorů: Department of Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan, United States of America aff001;  Division of Gastroenterology, Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan, United States of America aff002;  Department of Pediatrics, Washington University, St. Louis, Missouri, United States of America aff003;  St. Jude Children’s Hospital, Memphis, Tennessee, United States of America aff004;  Enteric Virus Laboratory, Department of Genetics, Microbiology and Statistics, University of Barcelona, Barcelona, Spain aff005;  Division of Virology, Department of Pathology, University of Cambridge, Addenbrooke's Hospital, Cambridge, United Kingdom aff006;  Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, Michigan, United States of America aff007;  Department of Biomedical Engineering, University of Michigan, Ann arbor, Michigan, United States of America aff008;  Departments of Molecular Microbiology, and Pathology and Immunology, Washington University, St. Louis, Missouri, United States of America aff009
Vyšlo v časopise: Astrovirus replication in human intestinal enteroids reveals multi-cellular tropism and an intricate host innate immune landscape. PLoS Pathog 15(10): e32767. doi:10.1371/journal.ppat.1008057
Kategorie: Research Article
doi: 10.1371/journal.ppat.1008057

Souhrn

Human astroviruses (HAstV) are understudied positive-strand RNA viruses that cause gastroenteritis mostly in children and the elderly. Three clades of astroviruses, classic, MLB-type and VA-type have been reported in humans. One limitation towards a better understanding of these viruses has been the lack of a physiologically relevant cell culture model that supports growth of all clades of HAstV. Herein, we demonstrate infection of HAstV strains belonging to all three clades in epithelium-only human intestinal enteroids (HIE) isolated from biopsy-derived intestinal crypts. A detailed investigation of infection of VA1, a member of the non-canonical HAstV-VA/HMO clade, showed robust replication in HIE derived from different patients and from different intestinal regions independent of the cellular differentiation status. Flow cytometry and immunofluorescence analysis revealed that VA1 infects several cell types, including intestinal progenitor cells and mature enterocytes, in HIE cultures. RNA profiling of VA1-infected HIE uncovered that the host response to infection is dominated by interferon (IFN)-mediated innate immune responses. A comparison of the antiviral host response in non-transformed HIE and transformed human colon carcinoma Caco-2 cells highlighted significant differences between these cells, including an increased magnitude of the response in HIE. Additional studies confirmed the sensitivity of VA1 to exogenous IFNs, and the endogenous IFN response of HIE to curtail the growth of strains from all three clades. Genotypic variation in the permissiveness of different HIE lines to HAstV could be overcome by pharmacologic inhibition of JAK/STAT signaling. Collectively, our data identify HIE as a universal infection model for HAstV and an improved model of the intestinal epithelium to investigate enteric virus-host interactions.

Klíčová slova:

Caco-2 cells – Cell differentiation – Gastrointestinal tract – Interferons – RNA extraction – Viral replication – Rotavirus infection – Astrovirus infection


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Štítky
Hygiena a epidemiologie Infekční lékařství Laboratoř

Článek vyšel v časopise

PLOS Pathogens


2019 Číslo 10

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