The interferon stimulated gene 20 protein (ISG20) is an innate defense antiviral factor that discriminates self versus non-self translation

Autoři: Nannan Wu aff001;  Xuan-Nhi Nguyen aff001;  Li Wang aff002;  Romain Appourchaux aff001;  Chengfei Zhang aff002;  Baptiste Panthu aff001;  Henri Gruffat aff001;  Chloé Journo aff001;  Sandrine Alais aff001;  Juliang Qin aff002;  Na Zhang aff002;  Kevin Tartour aff001;  Frédéric Catez aff004;  Renaud Mahieux aff001;  Theophile Ohlmann aff001;  Mingyao Liu aff002;  Bing Du aff002;  Andrea Cimarelli aff001
Působiště autorů: CIRI, Centre International de Recherche en Infectiologie, Univ Lyon, Inserm, U1111, Université Claude Bernard Lyon 1, CNRS, UMR5308, ENS de Lyon, Lyon, France aff001;  Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Shanghai, China aff002;  Shanghai Emerging and Reemerging Infectious Disease Institute, Shanghai Public Health Clinical Center, Fudan University, Shanghai, China aff003;  Université de Lyon, Université Claude Bernard Lyon 1, INSERM 1052, CNRS 5286, Centre Léon Bérard, Cancer Research Center of Lyon, Lyon, France aff004
Vyšlo v časopise: The interferon stimulated gene 20 protein (ISG20) is an innate defense antiviral factor that discriminates self versus non-self translation. PLoS Pathog 15(10): e32767. doi:10.1371/journal.ppat.1008093
Kategorie: Research Article


ISG20 is a broad spectrum antiviral protein thought to directly degrade viral RNA. However, this mechanism of inhibition remains controversial. Using the Vesicular Stomatitis Virus (VSV) as a model RNA virus, we show here that ISG20 interferes with viral replication by decreasing protein synthesis in the absence of RNA degradation. Importantly, we demonstrate that ISG20 exerts a translational control over a large panel of non-self RNA substrates including those originating from transfected DNA, while sparing endogenous transcripts. This activity correlates with the protein’s ability to localize in cytoplasmic processing bodies. Finally, these functions are conserved in the ISG20 murine ortholog, whose genetic ablation results in mice with increased susceptibility to viral infection. Overall, our results posit ISG20 as an important defense factor able to discriminate the self/non-self origins of the RNA through translation modulation.

Klíčová slova:

Graphs – Interferons – Luciferase – Messenger RNA – Protein translation – RNA viruses – Transfection – Viral replication


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