Cell autonomous and non-autonomous functions of plant intracellular immune receptors in stomatal defense and apoplastic defense

Autoři: Jiapei Yan aff001;  Huiyun Yu aff001;  Bo Li aff003;  Anqi Fan aff001;  Jeffrey Melkonian aff005;  Xiue Wang aff004;  Tong Zhou aff002;  Jian Hua aff001
Působiště autorů: School of Integrative Plant Science, Plant Biology Section, Cornell University, Ithaca, NY, United States of America aff001;  Key Laboratory of Food Quality and Safety, Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing, China aff002;  School of Applied Physics and Engineering, Cornell University, Ithaca, NY, United States of America aff003;  State Key Lab of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, China aff004;  School of Integrative Plant Science, Crop and Soil Sciences, Cornell University, Ithaca, NY, United States of America aff005
Vyšlo v časopise: Cell autonomous and non-autonomous functions of plant intracellular immune receptors in stomatal defense and apoplastic defense. PLoS Pathog 15(10): e32767. doi:10.1371/journal.ppat.1008094
Kategorie: Research Article
doi: 10.1371/journal.ppat.1008094


Stomatal closure defense and apoplastic defense are two major immunity mechanisms restricting the entry and propagation of microbe pathogens in plants. Surprisingly, activation of plant intracellular immune receptor NLR genes, while enhancing whole plant disease resistance, were sometimes linked to a defective stomatal defense in autoimmune mutants. Here we report the use of high temperature and genetic chimera to investigate the inter-dependence of stomatal and apoplastic defenses in autoimmunity. High temperature inhibits both stomatal and apoplastic defenses in the wild type, and it suppresses constitutive apoplastic defense responses and rescues the deficiency of stomatal closure response in autoimmune mutants. Chimeric plants have been generated to activate NLR only in guard cells or the non-guard cells. NLR activation in guard cells inhibits stomatal closure defense response in a cell autonomous manner likely through repressing ABA responses. At the same time, it leads to increased whole plant resistance accompanied by a slight increase in apoplastic defense. In addition, NLR activation in both guard and non-guard cells affects stomatal aperture and water potential. This study thus reveals that NLR activation has a differential effect on immunity in a cell type specific matter, which adds another layer of immune regulation with spatial information.

Klíčová slova:

Gene expression – Genetically modified plants – Leaves – Plant pathogens – Stomata – Guard cells – Mesophyll cells – Plant disease resistance


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