A Pseudomonas aeruginosa type VI secretion system regulated by CueR facilitates copper acquisition

Autoři: Yuying Han aff001;  Tietao Wang aff001;  Gukui Chen aff001;  Qinqin Pu aff002;  Qiong Liu aff003;  Yani Zhang aff001;  Linghui Xu aff003;  Min Wu aff002;  Haihua Liang aff001
Působiště autorů: Key Laboratory of Resources Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi'an, ShaanXi, China aff001;  Department of Basic Science, School of Medicine and Health Science, University of North Dakota, Grand Forks, North Dakota, United States of America aff002;  Guangdong Province Key Laboratory of Microbial Signals and Disease Control, Integrative Microbiology Research Centre, South China Agricultural University, Guangzhou, GuangDong, China aff003
Vyšlo v časopise: A Pseudomonas aeruginosa type VI secretion system regulated by CueR facilitates copper acquisition. PLoS Pathog 15(12): e32767. doi:10.1371/journal.ppat.1008198
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.ppat.1008198


The type VI secretion system (T6SS) is widely distributed in Gram-negative bacteria, whose function is known to translocate substrates to eukaryotic and prokaryotic target cells to cause host damage or as a weapon for interbacterial competition. Pseudomonas aeruginosa encodes three distinct T6SS clusters (H1-, H2-, and H3-T6SS). The H1-T6SS-dependent substrates have been identified and well characterized; however, only limited information is available for the H2- and H3-T6SSs since relatively fewer substrates for them have yet been established. Here, we obtained P. aeruginosa H2-T6SS-dependent secretomes and further characterized the H2-T6SS-dependent copper (Cu2+)-binding effector azurin (Azu). Our data showed that both azu and H2-T6SS were repressed by CueR and were induced by low concentrations of Cu2+. We also identified the Azu-interacting partner OprC, a Cu2+-specific TonB-dependent outer membrane transporter. Similar to H2-T6SS genes and azu, expression of oprC was directly regulated by CueR and was induced by low Cu2+. In addition, the Azu-OprC-mediated Cu2+ transport system is critical for P. aeruginosa cells in bacterial competition and virulence. Our findings provide insights for understanding the diverse functions of T6SSs and the role of metal ions for P. aeruginosa in bacteria-bacteria competition.

Klíčová slova:

Glutathione chromatography – Outer membrane proteins – Plasmid construction – Polymerase chain reaction – Pseudomonas aeruginosa – Secretion – Secretion systems – Signal peptides


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