G protein α subunit suppresses sporangium formation through a serine/threonine protein kinase in Phytophthora sojae


Autoři: Min Qiu aff001;  Yaning Li aff001;  Xin Zhang aff001;  Mingrun Xuan aff001;  Baiyu Zhang aff001;  Wenwu Ye aff001;  Xiaobo Zheng aff001;  Francine Govers aff003;  Yuanchao Wang aff001
Působiště autorů: Department of Plant Pathology, Nanjing Agricultural University, Nanjing, Jiangsu, China aff001;  The Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education), Nanjing, Jiangsu, China aff002;  Laboratory of Phytopathology, Wageningen University, Wageningen, The Netherlands aff003
Vyšlo v časopise: G protein α subunit suppresses sporangium formation through a serine/threonine protein kinase in Phytophthora sojae. PLoS Pathog 16(1): e32767. doi:10.1371/journal.ppat.1008138
Kategorie: Research Article
doi: 10.1371/journal.ppat.1008138

Souhrn

Eukaryotic heterotrimeric guanine nucleotide-binding proteins consist of α, β, and γ subunits, which act as molecular switches to regulate a number of fundamental cellular processes. In the oomycete pathogen Phytophthora sojae, the sole G protein α subunit (Gα; encoded by PsGPA1) has been found to be involved in zoospore mobility and virulence, but how it functions remains unclear. In this study, we show that the Gα subunit PsGPA1 directly interacts with PsYPK1, a serine/threonine protein kinase that consists of an N-terminal region with unknown function and a C-terminal region with a conserved catalytic kinase domain. We generated knockout and knockout-complemented strains of PsYPK1 and found that deletion of PsYPK1 resulted in a pronounced reduction in the production of sporangia and oospores, in mycelial growth on nutrient poor medium, and in virulence. PsYPK1 exhibits a cytoplasmic-nuclear localization pattern that is essential for sporangium formation and virulence of P. sojae. Interestingly, PsGPA1 overexpression was found to prevent nuclear localization of PsYPK1 by exclusively binding to the N-terminal region of PsYPK1, therefore accounting for its negative role in sporangium formation. Our data demonstrate that PsGPA1 negatively regulates sporangium formation by repressing the nuclear localization of its downstream kinase PsYPK1.

Klíčová slova:

G-protein signaling – Gene expression – Hypocotyl – Mycelium – Phytophthora – Plant pathogens – Protein kinases – Soybean


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