Mutations in PIH proteins MOT48, TWI1 and PF13 define common and unique steps for preassembly of each, different ciliary dynein

Autoři: Ryosuke Yamamoto aff001;  Shiho Yanagi aff001;  Masahito Nagao aff001;  Yuya Yamasaki aff001;  Yui Tanaka aff001;  Winfield S. Sale aff002;  Toshiki Yagi aff003;  Takahide Kon aff001
Působiště autorů: Department of Biological Sciences, Graduate School of Science, Osaka University, Osaka, Japan aff001;  Department of Cell Biology, School of Medicine, Emory University, Atlanta, Georgia, United States of America aff002;  Faculty of Life and Environmental Sciences, Prefectural University of Hiroshima, Shobara, Hiroshima, Japan aff003
Vyšlo v časopise: Mutations in PIH proteins MOT48, TWI1 and PF13 define common and unique steps for preassembly of each, different ciliary dynein. PLoS Genet 16(11): e1009126. doi:10.1371/journal.pgen.1009126
Kategorie: Research Article
doi: 10.1371/journal.pgen.1009126


Ciliary dyneins are preassembled in the cytoplasm before being transported into cilia, and a family of proteins containing the PIH1 domain, PIH proteins, are involved in the assembly process. However, the functional differences and relationships between members of this family of proteins remain largely unknown. Using Chlamydomonas reinhardtii as a model, we isolated and characterized two novel Chlamydomonas PIH preassembly mutants, mot48-2 and twi1-1. A new allele of mot48 (ida10), mot48-2, shows large defects in ciliary dynein assembly in the axoneme and altered motility. A second mutant, twi1-1, shows comparatively smaller defects in motility and dynein assembly. A double mutant mot48-2; twi1-1 displays greater reduction in motility and in dynein assembly compared to each single mutant. Similarly, a double mutant twi1-1; pf13 also shows a significantly greater defect in motility and dynein assembly than either parent mutant. Thus, MOT48 (IDA10), TWI1 and PF13 may define different steps, and have partially overlapping functions, in a pathway required for ciliary dynein preassembly. Together, our data suggest the three PIH proteins function in preassembly steps that are both common and unique for different ciliary dyneins.

Klíčová slova:

Cilia – Cytoplasm – Dyneins – Chlamydomonas reinhardtii – Immunoblotting – Motor proteins – Phenotypes – Swimming


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