Meiotic gene silencing complex MTREC/NURS recruits the nuclear exosome to YTH-RNA-binding protein Mmi1

Autoři: Yuichi Shichino aff001;  Yoko Otsubo aff001;  Masayuki Yamamoto aff001;  Akira Yamashita aff001
Působiště autorů: Laboratory of Cell Responses, National Institute for Basic Biology, Nishigonaka, Myodaiji, Okazaki, Aichi, Japan aff001;  Laboratory of Cell Responses, National Institute for Basic Biology, Myodaiji, Okazaki, Aichi, Japan aff001;  National Institute for Fusion Science,Oroshi, Toki, Gifu, Japan aff002;  National Institute for Fusion Science, Toki, Gifu, Japan aff002;  Center for Novel Science Initiatives, National Institutes of Natural Sciences, Nishigonaka, Myodaiji, Okazaki, Aichi, Japan aff003;  Center for Novel Science Initiatives, National Institutes of Natural Sciences, Myodaiji, Okazaki, Aichi, Japan aff003;  Department of Basic Biology, School of Life Science, SOKENDAI (The Graduate University for Advanced Studies), Nishigonaka, Myodaiji, Okazaki, Aichi, Japan aff004;  Department of Basic Biology, School of Life Science, SOKENDAI (The Graduate University for Advanced Studies), Myodaiji, Okazaki, Aichi, Japan aff004
Vyšlo v časopise: Meiotic gene silencing complex MTREC/NURS recruits the nuclear exosome to YTH-RNA-binding protein Mmi1. PLoS Genet 16(2): e32767. doi:10.1371/journal.pgen.1008598
Kategorie: Research Article


Accurate target recognition in transcript degradation is crucial for regulation of gene expression. In the fission yeast Schizosaccharomyces pombe, a number of meiotic transcripts are recognized by a YTH-family RNA-binding protein, Mmi1, and selectively degraded by the nuclear exosome during mitotic growth. Mmi1 forms nuclear foci in mitotically growing cells, and the nuclear exosome colocalizes to such foci. However, it remains elusive how Mmi1 and the nuclear exosome are connected. Here, we show that a complex called MTREC (Mtl1-Red1 core) or NURS (nuclear RNA silencing) that consists of a zinc-finger protein, Red1, and an RNA helicase, Mtl1, is required for the recruitment of the nuclear exosome to Mmi1 foci. Physical interaction between Mmi1 and the nuclear exosome depends on Red1. Furthermore, a chimeric protein involving Mmi1 and Rrp6, which is a nuclear-specific component of the exosome, suppresses the ectopic expression phenotype of meiotic transcripts in red1Δ cells and mtl1 mutant cells. These data indicate that the primary function of MTREC/NURS in meiotic transcript elimination is to link Mmi1 to the nuclear exosome physically.

Klíčová slova:

Co-immunoprecipitation – Exosomes – Gene expression – Immunoprecipitation – Messenger RNA – Plasmid construction – Schizosaccharomyces pombe – Yellow fluorescent protein


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