Parallel and nonparallel genomic responses contribute to herbicide resistance in Ipomoea purpurea, a common agricultural weed


Autoři: Megan Van Etten aff001;  Kristin M. Lee aff002;  Shu-Mei Chang aff003;  Regina S. Baucom aff004
Působiště autorů: Biology Department, Penn State-Scranton, Dunmore, Pennsylvania, United States of America aff001;  Department of Biological Sciences, Columbia University, New York, New York, United States of America aff002;  Plant Biology Department, University of Georgia, Athens, Georgia, United States of America aff003;  Department of Ecology and Evolutionary Biology, University of Michigan, Ann Arbor, Michigan, United States of America aff004
Vyšlo v časopise: Parallel and nonparallel genomic responses contribute to herbicide resistance in Ipomoea purpurea, a common agricultural weed. PLoS Genet 16(2): e32767. doi:10.1371/journal.pgen.1008593
Kategorie: Research Article
doi: 10.1371/journal.pgen.1008593

Souhrn

The repeated evolution of herbicide resistance has been cited as an example of genetic parallelism, wherein separate species or genetic lineages utilize the same genetic solution in response to selection. However, most studies that investigate the genetic basis of herbicide resistance examine the potential for changes in the protein targeted by the herbicide rather than considering genome-wide changes. We used a population genomics screen and targeted exome re-sequencing to uncover the potential genetic basis of glyphosate resistance in the common morning glory, Ipomoea purpurea, and to determine if genetic parallelism underlies the repeated evolution of resistance across replicate resistant populations. We found no evidence for changes in 5‐enolpyruvylshikimate‐3‐phosphate synthase (EPSPS), glyphosate’s target protein, that were associated with resistance, and instead identified five genomic regions that showed evidence of selection. Within these regions, genes involved in herbicide detoxification—cytochrome P450s, ABC transporters, and glycosyltransferases—are enriched and exhibit signs of selective sweeps. One region under selection shows parallel changes across all assayed resistant populations whereas other regions exhibit signs of divergence. Thus, while it appears that the physiological mechanism of resistance in this species is likely the same among resistant populations, we find patterns of both similar and divergent selection across separate resistant populations at particular loci.

Klíčová slova:

Detoxification – Evolutionary genetics – Genetic loci – Haplotypes – Herbicides – Molecular genetics – Population genetics – Sequence assembly tools


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