Identifying neural substrates of competitive interactions and sequence transitions during mechanosensory responses in Drosophila

English version

Autoři: Jean-Baptiste Masson ^aff001; Francois Laurent ^aff002; Albert Cardona ^aff001; Chloe Barre ^aff002; Nicolas Skatchkovsky ^aff002; Marta Zlatic ^aff001; Tihana Jovanic ^aff001
Působiště autorů: Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, United States of America ^aff001; Decision and Bayesian Computation, USR 3756 (C3BI/DBC) & Neuroscience Department, Institut Pasteur & CNRS, Paris, France ^aff002; Department of Physiology, Development, and Neuroscience, Cambridge University, Cambridge, United Kingdom ^aff003; MRC Laboratory of Molecular Biology, Trumpington, Cambridge, United Kingdom ^aff004; Department of Zoology, Cambridge University, Cambridge, United Kingdom ^aff005; Université Paris-Saclay, CNRS, Institut des Neurosciences Paris Saclay, Gif-sur-Yvette, France ^aff006
Vyšlo v časopise: Identifying neural substrates of competitive interactions and sequence transitions during mechanosensory responses in Drosophila. PLoS Genet 16(2): e32767. doi:10.1371/journal.pgen.1008589
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pgen.1008589

Souhrn

Nervous systems have the ability to select appropriate actions and action sequences in response to sensory cues. The circuit mechanisms by which nervous systems achieve choice, stability and transitions between behaviors are still incompletely understood. To identify neurons and brain areas involved in controlling these processes, we combined a large-scale neuronal inactivation screen with automated action detection in response to a mechanosensory cue in Drosophila larva. We analyzed behaviors from 2.9x10⁵ larvae and identified 66 candidate lines for mechanosensory responses out of which 25 for competitive interactions between actions. We further characterize in detail the neurons in these lines and analyzed their connectivity using electron microscopy. We found the neurons in the mechanosensory network are located in different regions of the nervous system consistent with a distributed model of sensorimotor decision-making. These findings provide the basis for understanding how selection and transition between behaviors are controlled by the nervous system.

Klíčová slova:

Decision making – Drosophila melanogaster – Electron microscopy – Larvae – Motor neurons – Nervous system – Neurons – Sensory neurons

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