Quorum sensing sets the stage for the establishment and vertical transmission of Sodalis praecaptivus in tsetse flies

Autoři: Miguel Medina Munoz aff001;  Noah Spencer aff001;  Shinichiro Enomoto aff002;  Colin Dale aff002;  Rita V. M. Rio aff001
Působiště autorů: Department of Biology, Eberly College of Arts and Sciences, West Virginia University, Morgantown, WV, United States of America aff001;  Department of Biology, University of Utah, Salt Lake City, UT, United States of America aff002
Vyšlo v časopise: Quorum sensing sets the stage for the establishment and vertical transmission of Sodalis praecaptivus in tsetse flies. PLoS Genet 16(8): e32767. doi:10.1371/journal.pgen.1008992
Kategorie: Research Article
doi: 10.1371/journal.pgen.1008992


Bacterial virulence factors facilitate host colonization and set the stage for the evolution of parasitic and mutualistic interactions. The Sodalis-allied clade of bacteria exhibit striking diversity in the range of both plant and animal feeding insects they inhabit, suggesting the appropriation of universal molecular mechanisms that facilitate establishment. Here, we report on the infection of the tsetse fly by free-living Sodalis praecaptivus, a close relative of many Sodalis-allied symbionts. Key genes involved in quorum sensing, including the homoserine lactone synthase (ypeI) and response regulators (yenR and ypeR) are integral for the benign colonization of S. praecaptivus. Mutants lacking ypeI, yenR and ypeR compromised tsetse survival as a consequence of their inability to repress virulence. Genes under quorum sensing, including homologs of the binary insecticidal toxin PirAB and a putative symbiosis-promoting factor CpmAJ, demonstrated negative and positive impacts, respectively, on tsetse survival. Taken together with results obtained from experiments involving weevils, this work shows that quorum sensing virulence suppression plays an integral role in facilitating the establishment of Sodalis-allied symbionts in diverse insect hosts. This knowledge contributes to the understanding of the early evolutionary steps involved in the formation of insect-bacterial symbiosis. Further, despite having no established history of interaction with tsetse, S. praecaptivus can infect reproductive tissues, enabling vertical transmission through adenotrophic viviparity within a single host generation. This creates an option for the use of S. praecaptivus in the biocontrol of insect disease vectors via paratransgenesis.

Klíčová slova:

Insect vectors – Insects – Microinjection – Symbiosis – Virulence factors – Weevils – Quorum sensing – Tsetse fly


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2020 Číslo 8

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