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Correction: Co-Evolution of Mitochondrial tRNA Import and Codon Usage Determines Translational Efficiency in the Green Alga Chlamydomonas


Authors: Thalia Salinas;  Francéline Duby;  Véronique Larosa;  Nadine Coosemans;  Nathalie Bonnefoy;  Patrick Motte;  Laurence Maréchal-Drouard;  Claire Remacle
Published in the journal: Correction: Co-Evolution of Mitochondrial tRNA Import and Codon Usage Determines Translational Efficiency in the Green Alga Chlamydomonas. PLoS Genet 16(3): e32767. doi:10.1371/journal.pgen.1008719
Category: Correction
doi: https://doi.org/10.1371/journal.pgen.1008719

After this article [1] was published, questions were raised about possible vertical discontinuities between lanes in Figs 1B, 1D and 5A. The original image data supporting these figures are provided here in S1 File.

All data reported in Fig 1B are from the same gel, but there is a splicing junction between the “T-ND4-LP” and “T-Cucob” results where lanes from the original gel were removed in preparing the figure. The authors have provided an updated version of Fig 1 and its legend here. In the updated Fig 1B, the image splicing junction is indicated by a blank area and the separated panels represent fragments of the same gel image (see S1 File).

As shown in S1 File, Fig 1D reflects contiguous data from the original image.

In Fig 5A, each panel reports data from the same exposure of the same northern blot, but for all panels except the Val AAC panel there are image splicing junctions between lanes 1 and 2, where cytosolic data from the original northern blots were removed in preparing the figure. The authors have provided an updated version of Fig 5 here. In the updated Fig 5A, image splicing junctions are indicated by blank area between lanes, and the G2, L, and Qmt panels have been replaced due to the unavailability of the raw image data for the original panels. The updates to Fig 5A do not affect the quantification shown in Fig 5B. Quantitative data supporting Fig 5B are in S2 File.

Raw data underlying all other results reported in the article are available upon request from the corresponding author, except for four phosphorimager images that are not accessible anymore.

The authors apologize for not indicating the splicing junctions clearly in the published article.

Fig. 1. Molecular characterization of the transformants.
Molecular characterization of the transformants.
Fig. 2. Analysis of the import status of mitochondrial tRNAs in T22-11 transformant.
Analysis of the import status of mitochondrial tRNAs in T22-11 transformant.

Supporting information

S1 File [pdf]
Original image data supporting Figs , and .

S2 File [pdf]
Quantitative data supporting .


Zdroje

1. Salinas T, Duby F, Larosa V, Coosemans N, Bonnefoy N, Motte P, et al. (2012) Co-Evolution of Mitochondrial tRNA Import and Codon Usage Determines Translational Efficiency in the Green Alga Chlamydomonas. PLoS Genet 8(9): e1002946. https://doi.org/10.1371/journal.pgen.1002946 23028354


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