Spastin mutations impair coordination between lipid droplet dispersion and reticulum


Autoři: Yoan Arribat aff001;  Dogan Grepper aff001;  Sylviane Lagarrigue aff001;  Timothy Qi aff002;  Sarah Cohen aff002;  Francesca Amati aff001
Působiště autorů: Aging and Muscle Metabolism Lab, Department of Biomedical Sciences, School of Biology and Medicine, University of Lausanne, Lausanne, Switzerland aff001;  Department of Cell Biology and Physiology, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States aff002;  Service of Endocrinology, Diabetology & Metabolism, Lausanne University Hospital and Lausanne University, Lausanne, Switzerland aff003
Vyšlo v časopise: Spastin mutations impair coordination between lipid droplet dispersion and reticulum. PLoS Genet 16(4): e32767. doi:10.1371/journal.pgen.1008665
Kategorie: Research Article
doi: 10.1371/journal.pgen.1008665

Souhrn

Lipid droplets (LD) are affected in multiple human disorders. These highly dynamic organelles are involved in many cellular roles. While their intracellular dispersion is crucial to ensure their function and other organelles-contact, underlying mechanisms are still unclear. Here we show that Spastin, one of the major proteins involved in Hereditary Spastic Paraplegia (HSP), controls LD dispersion. Spastin depletion in zebrafish affects metabolic properties and organelle dynamics. These functions are ensured by a conserved complex set of splice variants. M1 isoforms determine LD dispersion in the cell by orchestrating endoplasmic reticulum (ER) shape along microtubules (MTs). To further impact LD fate, Spastin modulates transcripts levels and subcellular location of other HSP key players, notably Seipin and REEP1. In pathological conditions, mutations in human Spastin M1 disrupt this mechanism and impacts LD network. Spastin depletion influences not only other key proteins but also modulates specific neutral lipids and phospholipids, revealing an impact on membrane and organelle components. Altogether our results show that Spastin and its partners converge in a common machinery that coordinates LD dispersion and ER shape along MTs. Any alteration of this system results in HSP clinical features and impacts lipids profile, thus opening new avenues for novel biomarkers of HSP.

Klíčová slova:

Cellular structures and organelles – Confocal microscopy – Embryos – HeLa cells – Lipids – Skeletal muscles – Tubulins – Zebrafish


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