The Drosophila actin nucleator DAAM is essential for left-right asymmetry


Autoři: Anil Chougule aff001;  François Lapraz aff001;  István Földi aff002;  Delphine Cerezo aff001;  József Mihály aff002;  Stéphane Noselli aff001
Působiště autorů: Université Côte D’Azur, CNRS, Inserm, iBV, Nice, France aff001;  Biological Research Centre, Hungarian Academy of Sciences, Institute of Genetics,Hungary aff002;  Biological Research Centre, Hungarian Academy of Sciences, Institute of Genetics, Hungary aff002
Vyšlo v časopise: The Drosophila actin nucleator DAAM is essential for left-right asymmetry. PLoS Genet 16(4): e32767. doi:10.1371/journal.pgen.1008758
Kategorie: Research Article
doi: 10.1371/journal.pgen.1008758

Souhrn

Left-Right (LR) asymmetry is essential for organ positioning, shape and function. Myosin 1D (Myo1D) has emerged as an evolutionary conserved chirality determinant in both Drosophila and vertebrates. However, the molecular interplay between Myo1D and the actin cytoskeleton underlying symmetry breaking remains poorly understood. To address this question, we performed a dual genetic screen to identify new cytoskeletal factors involved in LR asymmetry. We identified the conserved actin nucleator DAAM as an essential factor required for both dextral and sinistral development. In the absence of DAAM, organs lose their LR asymmetry, while its overexpression enhances Myo1D-induced de novo LR asymmetry. These results show that DAAM is a limiting, LR-specific actin nucleator connecting up Myo1D with a dedicated F-actin network important for symmetry breaking.

Klíčová slova:

Actins – Cytoskeleton – Drosophila melanogaster – Genetic screens – Genital anatomy – Phenotypes – Protein domains – RNA interference


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