Multi-strain Tn-Seq reveals common daptomycin resistance determinants in Staphylococcus aureus


Autoři: Kathryn A. Coe aff001;  Wonsik Lee aff001;  Madeleine C. Stone aff001;  Gloria Komazin-Meredith aff003;  Timothy C. Meredith aff001;  Yonatan H. Grad aff004;  Suzanne Walker aff001
Působiště autorů: Department of Microbiology, Harvard Medical School, Boston, Massachusetts, United States of America aff001;  School of Pharmacy, Sungkyunkwan University, Suwon, Republic of Korea aff002;  Department of Biochemistry and Molecular Biology, Pennsylvania State University, Pennsylvania, United States of America aff003;  Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Boston, Massachusetts, United States of America aff004;  Division of Infectious Diseases, Brigham and Women’s Hospital, Harvard Medical School, Boston, Massachusetts, United States of America aff005;  Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts, United States of America aff006
Vyšlo v časopise: Multi-strain Tn-Seq reveals common daptomycin resistance determinants in Staphylococcus aureus. PLoS Pathog 15(11): e32767. doi:10.1371/journal.ppat.1007862
Kategorie: Research Article
doi: 10.1371/journal.ppat.1007862

Souhrn

Antibiotic-resistant Staphylococcus aureus remains a leading cause of antibiotic resistance-associated mortality in the United States. Given the reality of multi-drug resistant infections, it is imperative that we establish and maintain a pipeline of new compounds to replace or supplement our current antibiotics. A first step towards this goal is to prioritize targets by identifying the genes most consistently required for survival across the S. aureus phylogeny. Here we report the first direct comparison of multiple strains of S. aureus via transposon sequencing. We show that mutant fitness varies by strain in key pathways, underscoring the importance of using more than one strain to differentiate between core and strain-dependent essential genes. We treated the libraries with daptomycin to assess whether the strain-dependent differences impact pathways important for survival. Despite baseline differences in gene importance, several pathways, including the lipoteichoic acid pathway, consistently promote survival under daptomycin exposure, suggesting core vulnerabilities that can be exploited to resensitize daptomycin-nonsusceptible isolates. We also demonstrate the merit of using transposons with outward-facing promoters capable of overexpressing nearby genes for identifying clinically-relevant gain-of-function resistance mechanisms. Together, the daptomycin vulnerabilities and resistance mechanisms support a mode of action with wide-ranging effects on the cell envelope and cell division. This work adds to a growing body of literature demonstrating the nuanced insights gained by comparing Tn-Seq results across multiple bacterial strains.

Klíčová slova:

Antibiotic resistance – Antibiotics – Genomic libraries – Genomic library construction – Methicillin-resistant Staphylococcus aureus – Polymerase chain reaction – Staphylococcus aureus – Transposable elements


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Revmatoidní artritida: včas a k cíli
Autoři: MUDr. Heřman Mann

Jistoty a nástrahy antikoagulační léčby aneb kardiolog - neurolog - farmakolog - nefrolog - právník diskutují
Autoři: doc. MUDr. Štěpán Havránek, Ph.D., prof. MUDr. Roman Herzig, Ph.D., doc. MUDr. Karel Urbánek, Ph.D., prim. MUDr. Jan Vachek, MUDr. et Mgr. Jolana Těšínová, Ph.D.

Léčba akutní pooperační bolesti
Autoři: doc. MUDr. Jiří Málek, CSc.

Nové antipsychotikum kariprazin v léčbě schizofrenie
Autoři: prof. MUDr. Cyril Höschl, DrSc., FRCPsych.

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