Regulation of macrophage activity by surface receptors contained within Borrelia burgdorferi-enriched phagosomal fractions


Autoři: Ana Carreras-González aff001;  Diego Barriales aff001;  Ainhoa Palacios aff001;  Marta Montesinos-Robledo aff001;  Nicolás Navasa aff001;  Mikel Azkargorta aff002;  Ainize Peña-Cearra aff001;  Julen Tomás-Cortázar aff001;  Iraide Escobes aff002;  Miguel Angel Pascual-Itoiz aff001;  Jana Hradiská aff003;  Jan Kopecký aff003;  David Gil-Carton aff004;  Rafael Prados-Rosales aff001;  Leticia Abecia aff001;  Estíbaliz Atondo aff001;  Itziar Martín aff001;  Aize Pellón aff001;  Félix Elortza aff002;  Héctor Rodríguez aff001;  Juan Anguita aff001
Působiště autorů: Inflammation and Macrophage Plasticity Laboratory, CIC bioGUNE, Derio, Bizkaia, Spain aff001;  Proteomics Platform, CIBERehd, ProteoRed-ISCIII, CIC bioGUNE, Derio, Bizkaia, Spain aff002;  Faculty of Science, University of South Bohemia, Branišovská, České Budějovice, Czech Republic aff003;  Structural Biology Unit, CIC bioGUNE, Derio, Bizkaia, Spain aff004;  Ikerbasque, Basque Foundation for Science, Bilbao, Bizkaia, Spain aff005
Vyšlo v časopise: Regulation of macrophage activity by surface receptors contained within Borrelia burgdorferi-enriched phagosomal fractions. PLoS Pathog 15(11): e32767. doi:10.1371/journal.ppat.1008163
Kategorie: Research Article
doi: 10.1371/journal.ppat.1008163

Souhrn

Macrophages mediate the elimination of pathogens by phagocytosis resulting in the activation of specific signaling pathways that lead to the production of cytokines, chemokines and other factors. Borrelia burgdorferi, the causative agent of Lyme disease, causes a wide variety of pro-inflammatory symptoms. The proinflammatory capacity of macrophages is intimately related to the internalization of the spirochete. However, most receptors mediating this process are largely unknown. We have applied a multiomic approach, including the proteomic analysis of B. burgdorferi-containing phagosome-enriched fractions, to identify surface receptors that are involved in the phagocytic capacity of macrophages as well as their inflammatory output. Sucrose gradient protein fractions of human monocyte-derived macrophages exposed to B. burgdorferi contained the phagocytic receptor, CR3/CD14 highlighting the major role played by these proteins in spirochetal phagocytosis. Other proteins identified in these fractions include C-type lectins, scavenger receptors or Siglecs, of which some are directly involved in the interaction with the spirochete. We also identified the Fc gamma receptor pathway, including the binding receptor, CD64, as involved both in the phagocytosis of, and TNF induction in response to B. burgdorferi in the absence of antibodies. The common gamma chain, FcγR, mediates the phagocytosis of the spirochete, likely through Fc receptors and C-type lectins, in a process that involves Syk activation. Overall, these findings highlight the complex array of receptors involved in the phagocytic response of macrophages to B. burgdorferi.

Klíčová slova:

Borrelia burgdorferi – Cell binding – Macrophages – Phagocytosis – Fc receptors – Spirochetes – Phagosomes – Complement receptors


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Štítky
Hygiena a epidemiologie Infekční lékařství Laboratoř

Článek vyšel v časopise

PLOS Pathogens


2019 Číslo 11

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