Effector gene reshuffling involves dispensable mini-chromosomes in the wheat blast fungus

Autoři: Zhao Peng aff001;  Ely Oliveira-Garcia aff001;  Guifang Lin aff001;  Ying Hu aff001;  Melinda Dalby aff001;  Pierre Migeon aff001;  Haibao Tang aff003;  Mark Farman aff004;  David Cook aff001;  Frank F. White aff002;  Barbara Valent aff001;  Sanzhen Liu aff001
Působiště autorů: Department of Plant Pathology, Kansas State University, Manhattan, KS, United States of America aff001;  Department of Plant Pathology, University of Florida, Gainesville, FL, United States of America aff002;  Center for Genomics and Biotechnology and Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Fujian Agriculture and Forestry University, Fujian, China aff003;  Department of Plant Pathology, University of Kentucky, Lexington, KY, United States of America aff004
Vyšlo v časopise: Effector gene reshuffling involves dispensable mini-chromosomes in the wheat blast fungus. PLoS Genet 15(9): e32767. doi:10.1371/journal.pgen.1008272
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pgen.1008272


Newly emerged wheat blast disease is a serious threat to global wheat production. Wheat blast is caused by a distinct, exceptionally diverse lineage of the fungus causing rice blast disease. Through sequencing a recent field isolate, we report a reference genome that includes seven core chromosomes and mini-chromosome sequences that harbor effector genes normally found on ends of core chromosomes in other strains. No mini-chromosomes were observed in an early field strain, and at least two from another isolate each contain different effector genes and core chromosome end sequences. The mini-chromosome is enriched in transposons occurring most frequently at core chromosome ends. Additionally, transposons in mini-chromosomes lack the characteristic signature for inactivation by repeat-induced point (RIP) mutation genome defenses. Our results, collectively, indicate that dispensable mini-chromosomes and core chromosomes undergo divergent evolutionary trajectories, and mini-chromosomes and core chromosome ends are coupled as a mobile, fast-evolving effector compartment in the wheat pathogen genome.

Klíčová slova:

Biology and life sciences – Organisms – Eukaryota – Plants – Grasses – Wheat – Rice – Fungi – Genetics – Genomics – Genome analysis – Sequence assembly tools – Mobile genetic elements – Transposable elements – Fungal genomics – Genetic elements – Fungal genetics – Computational biology – Mycology – Plant science – Plant pathology – Plant pathogens – Plant fungal pathogens – Rice blast fungus – Research and analysis methods – Animal studies – Experimental organism systems – Plant and algal models – Database and informatics methods – Bioinformatics – Sequence analysis – Sequence alignment


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