Reduction of mRNA export unmasks different tissue sensitivities to low mRNA levels during Caenorhabditis elegans development


Autoři: Angelina Zheleva aff001;  Eva Gómez-Orte aff001;  Beatriz Sáenz-Narciso aff001;  Begoña Ezcurra aff001;  Henok Kassahun aff002;  María de Toro aff001;  Antonio Miranda-Vizuete aff004;  Ralf Schnabel aff005;  Hilde Nilsen aff002;  Juan Cabello aff001
Působiště autorů: CIBIR (Center for Biomedical Research of La Rioja), Logroño, La Rioja, Spain aff001;  Department of Clinical Molecular Biology, Institute of Clinical Medicine, University of Oslo and Akershus University Hospital, Lørenskog, Norway aff002;  South-Eastern Norway Regional Health Authority, Hamar, Norway aff003;  Instituto de Biomedicina de Sevilla, Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla, Sevilla, Spain aff004;  Institute of Genetics, Technische Universität Braunschweig, Germany aff005
Vyšlo v časopise: Reduction of mRNA export unmasks different tissue sensitivities to low mRNA levels during Caenorhabditis elegans development. PLoS Genet 15(9): e32767. doi:10.1371/journal.pgen.1008338
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pgen.1008338

Souhrn

Animal development requires the execution of specific transcriptional programs in different sets of cells to build tissues and functional organs. Transcripts are exported from the nucleus to the cytoplasm where they are translated into proteins that, ultimately, carry out the cellular functions. Here we show that in Caenorhabditis elegans, reduction of mRNA export strongly affects epithelial morphogenesis and germline proliferation while other tissues remain relatively unaffected. Epithelialization and gamete formation demand a large number of transcripts in the cytoplasm for the duration of these processes. In addition, our findings highlight the existence of a regulatory feedback mechanism that activates gene expression in response to low levels of cytoplasmic mRNA. We expand the genetic characterization of nuclear export factor NXF-1 to other members of the mRNA export pathway to model mRNA export and recycling of NXF-1 back to the nucleus. Our model explains how mutations in genes involved in general processes, such as mRNA export, may result in tissue-specific developmental phenotypes.

Klíčová slova:

Biology and life sciences – Developmental biology – Embryology – Embryos – Organisms – Eukaryota – Animals – Invertebrates – Nematoda – Caenorhabditis – Caenorhabditis elegans – Genetics – Epigenetics – RNA interference – Gene expression – Genetic interference – Biochemistry – Nucleic acids – RNA – Messenger RNA – Cell biology – Cellular structures and organelles – Cytoplasm – Cell processes – Cell cycle and cell division – Anatomy – Digestive system – Pharynx – Respiratory system – Reproductive system – Genital anatomy – Gonads – Research and analysis methods – Animal studies – Experimental organism systems – Model organisms – Animal models – Medicine and health sciences


Zdroje

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