Behavioral and brain- transcriptomic synchronization between the two opponents of a fighting pair of the fish Betta splendens

Autoři: Trieu-Duc Vu aff001;  Yuki Iwasaki aff002;  Shuji Shigenobu aff007;  Akiko Maruko aff001;  Kenshiro Oshima aff001;  Erica Iioka aff002;  Chao-Li Huang aff008;  Takashi Abe aff009;  Satoshi Tamaki aff010;  Yi-Wen Lin aff005;  Chih-Kuan Chen aff004;  Mei-Yeh Lu aff004;  Masaru Hojo aff005;  Hao-Ven Wang aff005;  Shun-Fen Tzeng aff005;  Hao-Jen Huang aff005;  Akio Kanai aff010;  Takashi Gojobori aff011;  Tzen-Yuh Chiang aff005;  H. Sunny Sun aff012;  Wen-Hsiung Li aff004;  Norihiro Okada aff001
Působiště autorů: School of Pharmacy, Kitasato University, Tokyo, Japan aff001;  Foundation for Advancement of International Science, Tsukuba, Japan aff002;  Life Sciences and Biotechnology Dept, Tokyo Institute of Technology, Tokyo, Japan aff003;  Biodiversity Research Center, Academia Sinica, Taipei, Taiwan aff004;  Department of Life Sciences, National Cheng Kung University, Tainan, Taiwan aff005;  Nagahama Institute of Bio-Science and Technology, Nagahama, Japan aff006;  National Institute for Basic Biology, Okazaki, Japan aff007;  Institute of Tropical Plant Sciences, National Cheng Kung University, Tainan, Taiwan aff008;  Graduate School of Science and Technology, Niigata University, Niigata, Japan aff009;  Institute for Advanced Biosciences, Keio University, Yamagata, Japan aff010;  Computational Bioscience Research Center, King Abdullah University of Science and Technology, Thuwal, Kingdom of Saudi Arabia aff011;  Institute of Molecular Medicine, National Cheng Kung University, Tainan, Taiwan aff012;  Department of Ecology and Evolution, University of Chicago, IL, United States of America aff013
Vyšlo v časopise: Behavioral and brain- transcriptomic synchronization between the two opponents of a fighting pair of the fish Betta splendens. PLoS Genet 16(6): e32767. doi:10.1371/journal.pgen.1008831
Kategorie: Research Article
doi: 10.1371/journal.pgen.1008831


Conspecific male animals fight for resources such as food and mating opportunities but typically stop fighting after assessing their relative fighting abilities to avoid serious injuries. Physiologically, how the fighting behavior is controlled remains unknown. Using the fighting fish Betta splendens, we studied behavioral and brain-transcriptomic changes during the fight between the two opponents. At the behavioral level, surface-breathing, and biting/striking occurred only during intervals between mouth-locking. Eventually, the behaviors of the two opponents became synchronized, with each pair showing a unique behavioral pattern. At the physiological level, we examined the expression patterns of 23,306 brain transcripts using RNA-sequencing data from brains of fighting pairs after a 20-min (D20) and a 60-min (D60) fight. The two opponents in each D60 fighting pair showed a strong gene expression correlation, whereas those in D20 fighting pairs showed a weak correlation. Moreover, each fighting pair in the D60 group showed pair-specific gene expression patterns in a grade of membership analysis (GoM) and were grouped as a pair in the heatmap clustering. The observed pair-specific individualization in brain-transcriptomic synchronization (PIBS) suggested that this synchronization provides a physiological basis for the behavioral synchronization. An analysis using the synchronized genes in fighting pairs of the D60 group found genes enriched for ion transport, synaptic function, and learning and memory. Brain-transcriptomic synchronization could be a general phenomenon and may provide a new cornerstone with which to investigate coordinating and sustaining social interactions between two interacting partners of vertebrates.

Klíčová slova:

Animal behavior – Fish physiology – Gene expression – Learning and memory – MAPK signaling cascades – RNA sequencing – Transcriptome analysis – Zebrafish


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